LAB BOOK Newsletter

APPLICATION NOTES

ebolavirus

Virus Specimen Preparation in Biosafety Level 3-4 Laboratories

This application note describes the negative stain TEM preparation of pathogenic Ebola virus in high containment BSL4 labs. Scientists from US Army Medical Research Institute of Infectious Diseases (USAMRIID) used mPrep™/g capsules to eliminate grid handling in the BSL suite, resulting in easier, safer processing and more reproducible sample preparation.

DNA-RecA

Negative Staining of Macromolecular Specimen for TEM

This application note illustrates the preparation of macromolecular specimens for negative stain TEM using mPrep™/g capsules. The example is a DNA-RecA complex, although the protocol is applicable to many other specimens.

PUBLICATION  |  DIGITAL MEDIA

Blancett, C.D. et al. J Vis Exp. 2017; (125): 56122

Authors describe a technique designed specifically for use in BSL-3 or -4 biocontainment to ease sample preparation in any lab environment by enabling easy negative staining of virus. This same method can also be applied to prepare negative stained TEM specimens of nanoparticles, macromolecules and similar specimens.

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